diff --git a/pkgs/applications/video/streamlink/default.nix b/pkgs/applications/video/streamlink/default.nix
index 4abc2b6546aa..c82cfb47cc32 100644
--- a/pkgs/applications/video/streamlink/default.nix
+++ b/pkgs/applications/video/streamlink/default.nix
@@ -1,17 +1,16 @@
 { lib
 , python3Packages
-, rtmpdump
 , ffmpeg
 , fetchpatch
 }:
 
 python3Packages.buildPythonApplication rec {
   pname = "streamlink";
-  version = "2.4.0";
+  version = "3.0.1";
 
   src = python3Packages.fetchPypi {
     inherit pname version;
-    sha256 = "e95588e222d1a7bd51e3171cd4bce84fd6f646418537aff37993d40f597810af";
+    sha256 = "sha256-GDbUlu5tRL5mdT7KrIdmxCbeAtYL1xvZXyZFwiuvKXo=";
   };
 
   checkInputs = with python3Packages; [
@@ -22,26 +21,20 @@ python3Packages.buildPythonApplication rec {
   ];
 
   propagatedBuildInputs = (with python3Packages; [
-    pycryptodome
-    requests
-    iso-639
-    iso3166
-    websocket-client
     isodate
     lxml
+    pycountry
+    pycryptodome
+    pysocks
+    requests
+    websocket-client
   ]) ++ [
-    rtmpdump
     ffmpeg
   ];
 
-  patches = [
-    # Patch failing tests to expect correct Accept-Encoding as generated by Requests
-    (fetchpatch {
-      url = "https://github.com/streamlink/streamlink/commit/ae747a113199c119bced4613d33edcc67a222bb9.patch";
-      includes = [ "tests/test_stream_json.py" ];
-      sha256 = "sha256-KEgyWdh5DNgNktmLSvKQowUQO9p9Q7zP4NbCQJPNgKw=";
-    })
-  ];
+  postPatch = ''
+    substituteInPlace setup.cfg --replace 'lxml >=4.6.4,<5.0' 'lxml'
+  '';
 
   meta = with lib; {
     homepage = "https://streamlink.github.io/";