diff --git a/changelogs/fragments/ansible-test-nuke-venv-isolated.yml b/changelogs/fragments/ansible-test-nuke-venv-isolated.yml
new file mode 100644
index 00000000000..eb15d326969
--- /dev/null
+++ b/changelogs/fragments/ansible-test-nuke-venv-isolated.yml
@@ -0,0 +1,2 @@
+minor_changes:
+  - ansible-test - virtualenv-isolated.sh is no longer provided. Prefer virtualenv.sh in its place.
diff --git a/test/lib/ansible_test/_data/injector/virtualenv-isolated.sh b/test/lib/ansible_test/_data/injector/virtualenv-isolated.sh
deleted file mode 100644
index 316d094b3b3..00000000000
--- a/test/lib/ansible_test/_data/injector/virtualenv-isolated.sh
+++ /dev/null
@@ -1,18 +0,0 @@
-#!/usr/bin/env bash
-# Create and activate a fresh virtual environment with `source virtualenv-isolated.sh`.
-
-rm -rf "${OUTPUT_DIR}/venv"
-
-# Try to use 'venv' if it is available, then fallback to 'virtualenv' since some systems provide 'venv' although it is non-functional.
-if [[ "${ANSIBLE_TEST_PYTHON_VERSION}" =~ ^2\. ]] || ! "${ANSIBLE_TEST_PYTHON_INTERPRETER}" -m venv "${OUTPUT_DIR}/venv" > /dev/null 2>&1; then
-    rm -rf "${OUTPUT_DIR}/venv"
-    "${ANSIBLE_TEST_PYTHON_INTERPRETER}" -m virtualenv --python "${ANSIBLE_TEST_PYTHON_INTERPRETER}" "${OUTPUT_DIR}/venv"
-fi
-
-set +ux
-source "${OUTPUT_DIR}/venv/bin/activate"
-set -ux
-
-if [[ "${ANSIBLE_TEST_COVERAGE}" ]]; then
-    pip install coverage -c ../../../runner/requirements/constraints.txt --disable-pip-version-check
-fi