diff --git a/changelog.d/7448.misc b/changelog.d/7448.misc
new file mode 100644
index 0000000000..2163cc9b97
--- /dev/null
+++ b/changelog.d/7448.misc
@@ -0,0 +1 @@
+Remove storage method `get_hosts_in_room` that is no longer called anywhere.
\ No newline at end of file
diff --git a/synapse/storage/data_stores/main/roommember.py b/synapse/storage/data_stores/main/roommember.py
index d5bd0cb5cf..ae9a76713c 100644
--- a/synapse/storage/data_stores/main/roommember.py
+++ b/synapse/storage/data_stores/main/roommember.py
@@ -153,16 +153,6 @@ class RoomMemberWorkerStore(EventsWorkerStore):
                 self._check_safe_current_state_events_membership_updated_txn,
             )
 
-    @cachedInlineCallbacks(max_entries=100000, iterable=True, cache_context=True)
-    def get_hosts_in_room(self, room_id, cache_context):
-        """Returns the set of all hosts currently in the room
-        """
-        user_ids = yield self.get_users_in_room(
-            room_id, on_invalidate=cache_context.invalidate
-        )
-        hosts = frozenset(get_domain_from_id(user_id) for user_id in user_ids)
-        return hosts
-
     @cached(max_entries=100000, iterable=True)
     def get_users_in_room(self, room_id):
         return self.db.runInteraction(